Anti-NNMT Polyclonal Antibody (HW468014)

Name: Anti-NNMT Polyclonal Antibody
Brand: abinScience
SKU: HW468014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HW468014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NNMT (Met1-Arg258).

Target

Nicotinamide N-methyltransferase, NNMT

Purification

Purified by antigen affinity column.

Accession

P40261

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nicotinamide N-methyltransferase (NNMT) is a ~29 kDa protein. Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway. Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3. In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis.

1. Peng, Y. et al. (2011) Biochemistry 50, 7800-8. PMID: 21823666

2. Ulanovskaya, OA. et al. (2013) Nature chemical biology 9, 300-6. PMID: 23455543

3. Aksoy, S. et al. (1994) The Journal of biological chemistry 269, 14835-40. PMID: 8182091

4. Sperber, H. et al. (2015) Nature cell biology 17, 1523-35. PMID: 26571212

5. Eckert, MA. et al. (2019) Nature 569, 723-728. PMID: 31043742

6. Nemmara, VV. et al. (2018) ACS chemical biology 13, 2663-2672. PMID: 30044909

Note

For research use only.

Images

References

Datasheet

Anti-NNMT Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-NNMT Polyclonal Antibody (HW468014)

Overview

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References

Datasheet

SDS

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