Human NNMT Recombinant Protein (N-His) (HW468012)

Name: Human NNMT Recombinant Protein (N-His)
Brand: abinScience
SKU: HW468012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW468012
Description	Recombinant Human NNMT Protein, N-His (HW468012) expressed in E. coli, spanning Met1-Arg258. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P40261
Protein length	Met1-Arg258
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.04 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Nicotinamide N-methyltransferase, NNMT
Background	Nicotinamide N-methyltransferase (NNMT) is a ~29 kDa protein. Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway. Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3. In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. 1. Peng, Y. et al. (2011) Biochemistry 50, 7800-8. PMID: 21823666 2. Ulanovskaya, OA. et al. (2013) Nature chemical biology 9, 300-6. PMID: 23455543 3. Aksoy, S. et al. (1994) The Journal of biological chemistry 269, 14835-40. PMID: 8182091 4. Sperber, H. et al. (2015) Nature cell biology 17, 1523-35. PMID: 26571212 5. Eckert, MA. et al. (2019) Nature 569, 723-728. PMID: 31043742 6. Nemmara, VV. et al. (2018) ACS chemical biology 13, 2663-2672. PMID: 30044909
Note	For research use only.

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SDS-PAGE for Recombinant Human NNMT Protein, N-His

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Datasheet

Human NNMT Recombinant Protein (N-His).pdf

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Human NNMT Recombinant Protein (N-His) (HW468012)

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