Anti-Parkin Recombinant Antibody (N136A/18) (HT315023)

Name: Anti-Parkin Recombinant Antibody (N136A/18)
Brand: abinScience
SKU: HT315023
Price: 196 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT315023

Description

Anti-Parkin Recombinant Antibody (N136A/18) (HT315023) is a mouse monoclonal antibody detecting PRKN in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N136A/18

Target

Parkin, PARK2, Parkinson juvenile disease protein 2, E3 ubiquitin-protein ligase parkin, Parkin RBR E3 ubiquitin-protein ligase, PRKN, Parkinson disease protein 2

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-Parkin Antibody (N136A/18) (abinScience Cat# HT315023, RRID:AB_3728022)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

E3 ubiquitin-protein ligase parkin (PRKN) is a ~51 kDa protein. Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Substrates include SYT11 and VDAC1. Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2. Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation.

1. Wang, C. et al. (2018) Nature communications 9, 81. PMID: 29311685

2. Ham, SJ. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 4281-4291. PMID: 32047033

3. Shiba-Fukushima, K. et al. (2014) PLoS genetics 10, e1004861. PMID: 25474007

4. Kuroda, Y. et al. (2012) Human molecular genetics 21, 991-1003. PMID: 22082830

5. Yun, J. et al. (2014) eLife 3, e01958. PMID: 24898855

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Parkin Antibody (N136A/18)

References

Datasheet

Anti-Parkin Recombinant Antibody (N136A/18).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Parkin Recombinant Antibody (N136A/18) (HT315023)

Overview

Images

References

Datasheet

SDS