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Anti-PGF/PlGF Polyclonal Antibody (HW315014)

Anti-PGF/PlGF Polyclonal Antibody
Anti-PGF/PlGF Polyclonal Antibody
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Overview
Catalog No.HW315014
Description
Anti-PGF/PlGF Polyclonal Antibody (HW315014) is a rabbit polyclonal antibody detecting PLGF in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PGF/PlGF (Leu19-Arg131).
Target PLGF, PGF, PlGF, PGFL, Placenta growth factor
Purification Purified by antigen affinity column.
Accession P49763
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Placenta growth factor (PGF/PlGF) is a ~24 kDa protein. Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

Note For research use only.
Images
  • Anti-PGF/PlGF Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PGF/PlGF polyclonal antibody at 1ug/mL on recombinant PGF/PlGF (Catalog No. HW315012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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