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Anti-Phospho-Vimentin (pS34) Recombinant Antibody (YT33) (HY550023)

Anti-Phospho-Vimentin (pS34) Recombinant Antibody (YT33)
Anti-Phospho-Vimentin (pS34) Recombinant Antibody (YT33)
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Overview
Catalog No.HY550023
Species reactivityHuman
ApplicationsIHC, IP, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDYT33
Clonality Monoclonal
Target Vimentin, VIM
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P08670
RRID Anti-Phospho-Vimentin (pS34) Antibody (YT33) (abinScience Cat# HY550023, RRID:AB_3725747)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Vimentin (p-Vimentin) is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.

Note For research use only.
Images
  • Anti-Phospho-Vimentin (pS34) Recombinant Antibody (YT33)

    SDS-PAGE

    SDS-PAGE for Anti-Phospho-Vimentin (pS34) Antibody (YT33)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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