Anti-Phosphoserine-2-OMe Recombinant Antibody (N262/20) (HW732023)

Name: Anti-Phosphoserine-2-OMe Recombinant Antibody (N262/20)
Brand: abinScience
SKU: HW732023
Price: 196 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW732023

Description

Anti-Phosphoserine-2-OMe Recombinant Antibody (N262/20) (HW732023) is a mouse monoclonal antibody detecting PIP4K2A in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N262/20

Target

Phosphatidylinositol 5-Phosphate 4-Kinase, 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha, PtdIns(4)P-5-kinase B isoform, PI(5)P 4-kinase type II alpha, PIP5KIII, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Diphosphoinositide kinase 2-alpha, PI5P4Kalpha, PIP4KII-alpha, Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, PtdIns(5)P-4-kinase isoform 2-alpha, PIP4K2A, PtdIns(4)P-5-kinase C isoform, PI5P4KA, PIP5K2A, PIP5K2

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-Phosphoserine-2-OMe Antibody (N262/20) (abinScience Cat# HW732023, RRID:AB_3728242)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a ~46 kDa protein. Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Has both ATP- and GTP-dependent kinase activities. May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level.

1. Davis, MI. et al. (2013) PloS one 8, e54127. PMID: 23326584

2. Rameh, LE. et al. (1997) Nature 390, 192-6. PMID: 9367159

3. Sumita, K. et al. (2016) Molecular cell 61, 187-98. PMID: 26774281

4. Wilcox, A. et al. (2008) FEBS letters 582, 1391-4. PMID: 18364242

5. Hu, A. et al. (2018) Journal of lipid research 59, 507-514. PMID: 29353240

6. Wang, DG. et al. (2019) Cell reports 27, 1991-2001.e5. PMID: 31091439

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Phosphoserine-2-OMe Antibody (N262/20)

References

Datasheet

Anti-Phosphoserine-2-OMe Recombinant Antibody (N262/20).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Phosphoserine-2-OMe Recombinant Antibody (N262/20) (HW732023)

Overview

Images

References

Datasheet

SDS