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Anti-PhyH/PAHX Recombinant Antibody (N210/9) (HT174033)

Anti-PhyH/PAHX Recombinant Antibody (N210/9)
Anti-PhyH/PAHX Recombinant Antibody (N210/9)
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Overview
Catalog No.HT174033
Description
Anti-PhyH/PAHX Recombinant Antibody (N210/9) (HT174033) is a mouse monoclonal antibody detecting PHYH in IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
ApplicationsIHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDN210/9
Target PAHX, PhyH, Phytanoyl-CoA alpha-hydroxylase, Phytanic acid oxidase, PHYH, Phytanoyl-CoA dioxygenase, peroxisomal
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
RRID

Anti-PhyH/PAHX Antibody (N210/9) (abinScience Cat# HT174033, RRID:AB_3727985)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Phytanoyl-CoA dioxygenase, peroxisomal (PHYH) is a ~38 kDa protein. Catalyzes the 2-hydroxylation of not only racemic phytanoyl-CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms). Does not hydroxylate long and very long straight chain acyl-CoAs or 2-methyl- and 4-methyl-branched acyl-CoAs.

1. Croes, K. et al. (2000) Journal of lipid research 41, 629-36. PMID: 10744784
2. Mukherji, M. et al. (2002) Chemistry & biology 9, 597-605. PMID: 12031666
3. Foulon, V. et al. (2003) Journal of lipid research 44, 2349-55. PMID: 12923223
4. Mihalik, SJ. et al. (1997) Nature genetics 17, 185-9. PMID: 9326939
Note For research use only.
Images
  • Anti-PhyH/PAHX Recombinant Antibody (N210/9)

    SDS-PAGE

    SDS-PAGE for Anti-PhyH/PAHX Antibody (N210/9)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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