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Anti-Rice OsOMT1/COMT Polyclonal Antibody (PR013014)

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Overview
Catalog No.PR013014
Description
Anti-Rice OsOMT1/COMT Polyclonal Antibody (PR013014) is a rabbit polyclonal antibody detecting OsOMT1. Suitable for Oryza sativa subsp. japonica.
Species reactivityOryza sativa (Rice)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Rice OsOMT1/COMT (Ser3-Lys368).
Target Acetylserotonin O-methyltransferase COMT, COMT, Caffeate O-methyltransferase 1, EC:2.1.1.4, EC:2.1.1.42, EC:2.1.1.68, Flavone 3'-O-methyltransferase 1, OsCOMT, OsCOMT1, OsOMT1, Quercetin 3'-O-methyltransferase 1, ROMT-9
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6ZD89
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Flavone 3'-O-methyltransferase 1 (OsOMT1/COMT) is a ~39 kDa protein. Methylates OH residues of flavonoid compounds. Can methylate eriodictyol, luteolin, quercetin and taxifolin. Methylates caffeate to produce ferrulate (PubMed:21210840, Ref.16). Catalyzes the methylation of monolignols, the lignin precursors. Functions cooperatively with CAD2 in the culm internodes for the biosynthesis of monolignols.

1. Kim, BG. et al. (2006) Phytochemistry 67, 387-94. PMID: 16412485
2. Kang, K. et al. (2011) Journal of pineal research 50, 304-9. PMID: 21210840
3. Hirano, K. et al. (2012) Plant cell reports 31, 91-101. PMID: 21912859
4. Byeon, Y. et al. (2015) Journal of experimental botany 66, 6917-25. PMID: 26276868
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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