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Anti-SARS-CoV-2 Nucleoprotein Monoclonal Antibody (1A165) (VK805045)

Anti-SARS-CoV-2 Nucleoprotein Monoclonal Antibody (1A165)
Anti-SARS-CoV-2 Nucleoprotein Monoclonal Antibody (1A165)
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Overview
Catalog No.VK805045
Description
Anti-SARS-CoV-2 NP/Nucleoprotein Monoclonal Antibody (1A165) [1A165] (VK805045) is a mouse monoclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.
Highlights
  • ●Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • ●Multi-Application — Validated across multiple applications.
Species reactivitySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
ApplicationsELISA, IHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1
Clone ID1A165
Immunogen E. coli - derived recombinant SARS-CoV-2 N Protein (Met1-Ala419).
Target Nucleoprotein, N, Nucleocapsid protein, NC, Protein N, N Protein
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein (NP) is a ~45 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dinesh, DC. et al. (2020) PLoS pathogens 16, e1009100. PMID: 33264373
2. Nabeel-Shah, S. et al. (2022) iScience 25, 103562. PMID: 34901782
3. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661
Note For research use only.
Images
  • Anti-SARS-CoV-2 Nucleoprotein Monoclonal Antibody (1A165)

    SDS-PAGE

    SDS-PAGE for Anti-SARS-CoV-2 NP/Nucleoprotein Monoclonal Antibody (1A165)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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