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Anti-scFv Monoclonal Antibody (1A466) (YC148025)

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Anti-scFv Monoclonal Antibody (1A466)
Anti-scFv Monoclonal Antibody (1A466)
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Overview
Catalog No.YC148025
Description
Anti-scFv Monoclonal Antibody (1A466) [1A466] (YC148025) is a mouse monoclonal antibody detecting scFv in FCM, ELISA.
Highlights
  • ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivityGeneral
ApplicationsFCM, ELISA
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG
Clone ID1A466
Immunogen CHO - derived recombinant scFv.
Tested applications FCM
Target scFv
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

scFv is a protein.

Note For research use only.
Images
  • Anti-scFv Monoclonal Antibody (1A466)

    Flow cytometry

    Flow-cytometry using anti-scFv antibody. Transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-scFv monoclonal antibody (Catalog YC148025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-scFv Monoclonal Antibody (1A466)

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