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Anti-SNRPD1 Polyclonal Antibody (HX906014)

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Overview
Catalog No.HX906014
Description
Anti-SNRPD1 Polyclonal Antibody (HX906014) is a rabbit polyclonal antibody detecting SNRPD1 in ELISA, IHC, WB. Suitable for Bovine, Human, Macaca fascicularis, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SNRPD1 (Met1-Arg119).
Target Sm-D1, Small nuclear ribonucleoprotein Sm D1, SNRPD1, Sm-D autoantigen, snRNP core protein D1
Purification Purified by antigen affinity column.
Accession P62314
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Small nuclear ribonucleoprotein Sm D1 (SNRPD1) is a ~13 kDa protein. Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638
2. Chari, A. et al. (2008) Cell 135, 497-509. PMID: 18984161
3. Pomeranz Krummel, DA. et al. (2009) Nature 458, 475-80. PMID: 19325628
4. Grimm, C. et al. (2013) Molecular cell 49, 692-703. PMID: 23333303
5. Kondo, Y. et al. (2015) eLife 4. PMID: 25555158
6. Agafonov, DE. et al. (2016) Science (New York, N.Y.) 351, 1416-20. PMID: 26912367
7. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346
8. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770
9. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166
10. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006
Note For research use only.
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Formula
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Anti-SNRPD1 Polyclonal Antibody

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