Anti-TLR7 Polyclonal Antibody (HV024014)

Name: Anti-TLR7 Polyclonal Antibody
Brand: abinScience
SKU: HV024014
Price: 168 USD
Availability: InStock

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Overview
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Overview

Catalog No.

HV024014

Description

Anti-TLR7 Polyclonal Antibody (HV024014) is a rabbit polyclonal antibody detecting TLR7 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TLR7 (Leu24-Ser348).

Target

TLR7, Toll-like receptor 7

Purification

Purified by antigen affinity column.

Accession

Q9NYK1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Toll-like receptor 7 (TLR7) is a ~120 kDa protein. Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively. In plasmacytoid dendritic cells, RNASET2 endonuclease cooperates with PLD3 or PLD4 5'->3' exonucleases to process RNA and release 2',3'-cyclic guanosine monophosphate (2',3'-cGMP) and cytidine-rich RNA fragments that occupy TLR7 ligand-binding pockets and trigger a signaling-competent state.

1. Diebold, SS. et al. (2004) Science (New York, N.Y.) 303, 1529-31. PMID: 14976261

2. Heinz, LX. et al. (2020) Nature 581, 316-322. PMID: 32433612

3. Lee, J. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 6646-51. PMID: 12738885

4. Zhang, Z. et al. (2016) Immunity 45, 737-748. PMID: 27742543

5. Davenne, T. et al. (2020) European journal of immunology 50, 56-62. PMID: 31608988

6. van der Made, CI. et al. (2020) JAMA 324, 663-673. PMID: 32706371

7. Brown, GJ. et al. (2022) Nature 605, 349-356. PMID: 35477763

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TLR7 polyclonal antibody at 1ug/mL on recombinant TLR7 (Catalog No. HV024012).

References

Datasheet

Anti-TLR7 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-TLR7 Polyclonal Antibody (HV024014)

Overview

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References

Datasheet

SDS

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