Anti-TNFa/TNF-alpha Polyclonal Antibody (HF879014)

Name: Anti-TNFa/TNF-alpha Polyclonal Antibody
Brand: abinScience
SKU: HF879014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF879014

Description

Anti-TNFa/TNF-alpha Polyclonal Antibody (HF879014) is a rabbit polyclonal antibody detecting TNF-alpha in ELISA, IHC, WB. Suitable for Human, Dog, Cat, Callithrix jacchus, and Equus caballus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TNFa/TNF-alpha (Val77-Leu233).

Target

TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1

Purification

Purified by antigen affinity column.

Accession

P01375

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tumor necrosis factor (TNFa/TNF-alpha) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. TNFa is the therapeutic target of adalimumab (Humira).

1. Nie, H. et al. (2013) Nature medicine 19, 322-8. PMID: 23396208

2. Friedmann, E. et al. (2006) Nature cell biology 8, 843-8. PMID: 16829952

3. Jinesh G, G. et al. (2012) Journal of leukocyte biology 92, 233-44. PMID: 22517918

4. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TNFa/TNF-alpha polyclonal antibody at 1ug/mL on recombinant TNFa/TNF-alpha (Catalog No. HF879012).

References

Datasheet