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Anti-TOMM70 Polyclonal Antibody (HF954014)

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Overview
Catalog No.HF954014
Description
Anti-TOMM70 Polyclonal Antibody (HF954014) is a rabbit polyclonal antibody detecting TOMM70 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TOMM70 (Leu333-Leu608).
Target TOM70, Translocase of outer membrane 70 kDa subunit, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor, KIAA0719, TOMM70A, TOMM70, Translocase of outer mitochondrial membrane protein 70
Purification Purified by antigen affinity column.
Accession O94826
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mitochondrial import receptor subunit TOM70 (TOMM70) is a ~67 kDa protein. Acts as a receptor of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex). Recognizes and mediates the translocation of mitochondrial preproteins from the cytosol into the mitochondria in a chaperone dependent manner. Mediates TBK1 and IRF3 activation induced by MAVS in response to Sendai virus infection and promotes host antiviral responses during virus infection. Upon Sendai virus infection, recruits HSP90AA1:IRF3:BAX in mitochondrion and the complex induces apoptosis.

1. Young, JC. et al. (2003) Cell 112, 41-50. PMID: 12526792
2. Li, X. et al. (2022) Science (New York, N.Y.) 375, eabi4343. PMID: 35025629
3. Liu, XY. et al. (2010) Cell research 20, 994-1011. PMID: 20628368
4. Wei, B. et al. (2015) Journal of virology 89, 3804-18. PMID: 25609812
5. Jiang, HW. et al. (2020) Cellular & molecular immunology 17, 998-1000. PMID: 32728199
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-TOMM70 Polyclonal Antibody

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