Human TOMM70 Recombinant Protein (N-His) (HF954012)

Name: Human TOMM70 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF954012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF954012
Description	Recombinant Human TOMM70 Protein, N-His (HF954012) expressed in E. coli, spanning Leu333-Leu608. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O94826
Protein length	Leu333-Leu608
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.43 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	TOM70, Translocase of outer membrane 70 kDa subunit, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor, KIAA0719, TOMM70A, TOMM70, Translocase of outer mitochondrial membrane protein 70
Background	Mitochondrial import receptor subunit TOM70 (TOMM70) is a ~67 kDa protein. Acts as a receptor of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex). Recognizes and mediates the translocation of mitochondrial preproteins from the cytosol into the mitochondria in a chaperone dependent manner. Mediates TBK1 and IRF3 activation induced by MAVS in response to Sendai virus infection and promotes host antiviral responses during virus infection. Upon Sendai virus infection, recruits HSP90AA1:IRF3:BAX in mitochondrion and the complex induces apoptosis. 1. Young, JC. et al. (2003) Cell 112, 41-50. PMID: 12526792 2. Li, X. et al. (2022) Science (New York, N.Y.) 375, eabi4343. PMID: 35025629 3. Liu, XY. et al. (2010) Cell research 20, 994-1011. PMID: 20628368 4. Wei, B. et al. (2015) Journal of virology 89, 3804-18. PMID: 25609812 5. Jiang, HW. et al. (2020) Cellular & molecular immunology 17, 998-1000. PMID: 32728199
Note	For research use only.

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SDS-PAGE for Recombinant Human TOMM70 Protein, N-His

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Datasheet

Human TOMM70 Recombinant Protein (N-His).pdf

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Human TOMM70 Recombinant Protein (N-His) (HF954012)

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