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Anti-TPSAB1/Tryptase-1 Polyclonal Antibody (HS931014)

Anti-TPSAB1/Tryptase-1 Polyclonal Antibody
Anti-TPSAB1/Tryptase-1 Polyclonal Antibody
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Overview
Catalog No.HS931014
Description
Anti-TPSAB1/Tryptase-1 Polyclonal Antibody (HS931014) is a rabbit polyclonal antibody detecting Tryptase I in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TPSAB1/Tryptase-1 (Ile31-Pro275).
Target TPSB1, TPS2, Tryptase I, Tryptase-1, TPSAB1, Tryptase alpha-1, Tryptase alpha/beta-1, TPS1
Purification Purified by antigen affinity column.
Accession Q15661
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tryptase alpha/beta-1 (TPSAB1/Tryptase-1) is a ~30 kDa protein. Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates.

1. Jackson, NE. et al. (2008) The Journal of biological chemistry 283, 34178-87. PMID: 18854315
Note For research use only.
Images
  • Anti-TPSAB1/Tryptase-1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TPSAB1/Tryptase-1 polyclonal antibody at 1ug/mL on recombinant TPSAB1/Tryptase-1 (Catalog No. HS931012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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