Human TPSAB1/Tryptase-1 Recombinant Protein (C-His) (HS931011)

Name: Human TPSAB1/Tryptase-1 Recombinant Protein (C-His)
Brand: abinScience
SKU: HS931011
Price: 298 USD
Availability: InStock

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Overview
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Overview

Catalog No.	HS931011
Description	_x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Recombinant Human TPSAB1/Tryptase-1 Protein, C-His (HS931011) expressed in Mammalian Cells, spanning Met1-Pro275. Purity: >90% by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Highlights _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ ●His-Tagged — N-terminal 6×His tag for IMAC purification. _x005F_x000D_ ●Mammalian Expression — Proper folding and native glycosylation. _x005F_x000D_ ●High Purity — >90% purity verified by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_
Expression system	Mammalian cells
Accession	Q15661
Protein length	Met1-Pro275
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.28 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	TPSB1, TPS2, Tryptase I, Tryptase-1, TPSAB1, Tryptase alpha-1, Tryptase alpha/beta-1, TPS1
Background	Tryptase alpha/beta-1 (TPSAB1/Tryptase-1) is a ~30 kDa protein. Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates. _x005F_x000D_ _x005F_x000D_ 1. Jackson, NE. et al. (2008) The Journal of biological chemistry 283, 34178-87. PMID: 18854315 _x005F_x000D_
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human TPSAB1/Tryptase-1 Protein, C-His
Bioactivity

Detects Human β-tryptase/TPSAB1 Antibody (31A) in indirect ELISAs.
Western blot

Detected by Anti-TPSAB1/Tryptase-1 monoclonal antibody (Catalog No: HB801207)