Anti-TREK-1 K+ channel Recombinant Antibody (N252A/8) (HF823023)

Name: Anti-TREK-1 K+ channel Recombinant Antibody (N252A/8)
Brand: abinScience
SKU: HF823023
Price: 196 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF823023

Description

Anti-TREK-1 K+ channel Recombinant Antibody (N252A/8) (HF823023) is a mouse monoclonal antibody detecting KCNK2 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N252A/8

Target

KCNK2, Two pore domain potassium channel TREK-1, Potassium channel subfamily K member 2, Two pore potassium channel TPKC1, TREK, Outward rectifying potassium channel protein TREK-1, TREK1, TREK-1 K(+) channel subunit

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-TREK-1 K+ channel Antibody (N252A/8) (abinScience Cat# HF823023, RRID:AB_3728066)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Potassium channel subfamily K member 2 (KCNK2) is a ~47 kDa protein. K(+) channel that conducts voltage-dependent outward rectifying currents upon membrane depolarization. Voltage sensing is coupled to K(+) electrochemical gradient in an 'ion flux gating' mode where outward but not inward ion flow opens the gate. Converts to voltage-independent 'leak' conductance mode upon stimulation by various stimuli including mechanical membrane stretch, acidic pH, heat and lipids. Reversibly converts between a voltage-insensitive K(+) 'leak' channel and a voltage-dependent outward rectifying K(+) channel in a phosphorylation-dependent manner. Homo- and heterodimerizes to form functional channels with distinct regulatory and gating properties.

1. Patel, AJ. et al. (1999) Nature neuroscience 2, 422-6. PMID: 10321245

2. Meadows, HJ. et al. (2000) Pflugers Archiv : European journal of physiology 439, 714-22. PMID: 10784345

3. Bockenhauer, D. et al. (2001) Nature neuroscience 4, 486-91. PMID: 11319556

4. Plant, LD. et al. (2012) Science signaling 5, ra84. PMID: 23169818

5. Royal, P. et al. (2019) Neuron 101, 232-245.e6. PMID: 30573346

6. Sorum, B. et al. (2024) Nature communications 15, 3142. PMID: 38605031

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-TREK-1 K+ channel Antibody (N252A/8)

References

Datasheet

Anti-TREK-1 K+ channel Recombinant Antibody (N252A/8).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TREK-1 K+ channel Recombinant Antibody (N252A/8) (HF823023)

Overview

Images

References

Datasheet

SDS