Anti-TRPML3/Mucolipin-3 Recombinant Antibody (N268/5) (HA053033)

Name: Anti-TRPML3/Mucolipin-3 Recombinant Antibody (N268/5)
Brand: abinScience
SKU: HA053033
Price: 196 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HA053033

Description

Anti-TRPML3/Mucolipin-3 Recombinant Antibody (N268/5) (HA053033) is a mouse monoclonal antibody detecting TRPML3/Mucolipin-3 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N268/5

Target

MCOLN3, TRPML3, Transient receptor potential channel mucolipin 3, Mucolipin-3

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-TRPML3/Mucolipin-3 Antibody (N268/5) (abinScience Cat# HA053033, RRID:AB_3728589)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Mucolipin-3 is a ~64 kDa protein. Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as a Ca(2+)-permeable cation channel with inwardly rectifying activity. Mediates release of Ca(2+) from endosomes to the cytoplasm, contributes to endosomal acidification and is involved in the regulation of membrane trafficking and fusion in the endosomal pathway. Also permeable to Mg(2+), Na(+) and K(+). Does not seem to act as mechanosensory transduction channel in inner ear sensory hair cells.

1. Kim, HJ. et al. (2008) The EMBO journal 27, 1197-205. PMID: 18369318

2. Martina, JA. et al. (2009) Traffic (Copenhagen, Denmark) 10, 1143-56. PMID: 19497048

3. Kim, HJ. et al. (2009) Traffic (Copenhagen, Denmark) 10, 1157-67. PMID: 19522758

4. Curcio-Morelli, C. et al. (2010) Journal of cellular physiology 222, 328-35. PMID: 19885840

5. Zhou, X. et al. (2017) Nature structural & molecular biology 24, 1146-1154. PMID: 29106414

6. Lelouvier, B. et al. (2011) The Journal of biological chemistry 286, 9826-32. PMID: 21245134

7. Guo, Z. et al. (2013) PloS one 8, e58174. PMID: 23469151

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-TRPML3/Mucolipin-3 Antibody (N268/5)

References

Datasheet

Anti-TRPML3/Mucolipin-3 Recombinant Antibody (N268/5).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TRPML3/Mucolipin-3 Recombinant Antibody (N268/5) (HA053033)

Overview

Images

References

Datasheet

SDS