Anti-UBD Polyclonal Antibody (HT365014)

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Overview
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Datasheet
SDS

Overview

Catalog No.

HT365014

Description

Anti-UBD Polyclonal Antibody (HT365014) is a rabbit polyclonal antibody detecting UBD in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UBD (Met1-Gly165).

Target

Diubiquitin, UBD, Ubiquitin-like protein FAT10, Ubiquitin D, FAT10

Purification

Purified by antigen affinity column.

Accession

O15205

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin D (UBD) is a ~18 kDa protein. Ubiquitin-like protein modifier which can be covalently attached to target proteins and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner. Conjugation to the target protein is activated by UBA6 via adenylation of its C-terminal glycine. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs).

1. Hipp, MS. et al. (2005) Molecular and cellular biology 25, 3483-91. PMID: 15831455

2. Schmidtke, G. et al. (2006) The Journal of biological chemistry 281, 20045-54. PMID: 16707496

3. Schmidtke, G. et al. (2009) FEBS letters 583, 591-4. PMID: 19166848

4. Chiu, YH. et al. (2007) Molecular cell 27, 1014-23. PMID: 17889673

5. Truongvan, N. et al. (2022) Nature communications 13, 4789. PMID: 35970836

6. Gong, P. et al. (2010) Journal of the American Society of Nephrology : JASN 21, 316-26. PMID: 19959714

7. Ebstein, F. et al. (2009) The international journal of biochemistry & cell biology 41, 1205-15. PMID: 19028597

8. Ren, J. et al. (2006) The Journal of biological chemistry 281, 11413-21. PMID: 16495226

9. Kalveram, B. et al. (2008) Journal of cell science 121, 4079-88. PMID: 19033385

10. Ross, MJ. et al. (2006) Journal of the American Society of Nephrology : JASN 17, 996-1004. PMID: 16495380

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-UBD polyclonal antibody at 1ug/mL on recombinant UBD (Catalog No. HT365012).

References

Datasheet

Anti-UBD Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-UBD Polyclonal Antibody (HT365014)

Overview

Images

References

Datasheet

SDS

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