Human UBD Recombinant Protein (N-His) (HT365012)

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Overview

Catalog No.	HT365012
Description	Recombinant Human UBD Protein, N-His (HT365012) expressed in E. coli, spanning Met1-Gly165. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15205
Protein length	Met1-Gly165
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.63 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Diubiquitin, UBD, Ubiquitin-like protein FAT10, Ubiquitin D, FAT10
Background	Ubiquitin D (UBD) is a ~18 kDa protein. Ubiquitin-like protein modifier which can be covalently attached to target proteins and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner. Conjugation to the target protein is activated by UBA6 via adenylation of its C-terminal glycine. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). 1. Hipp, MS. et al. (2005) Molecular and cellular biology 25, 3483-91. PMID: 15831455 2. Schmidtke, G. et al. (2006) The Journal of biological chemistry 281, 20045-54. PMID: 16707496 3. Schmidtke, G. et al. (2009) FEBS letters 583, 591-4. PMID: 19166848 4. Chiu, YH. et al. (2007) Molecular cell 27, 1014-23. PMID: 17889673 5. Truongvan, N. et al. (2022) Nature communications 13, 4789. PMID: 35970836 6. Gong, P. et al. (2010) Journal of the American Society of Nephrology : JASN 21, 316-26. PMID: 19959714 7. Ebstein, F. et al. (2009) The international journal of biochemistry & cell biology 41, 1205-15. PMID: 19028597 8. Ren, J. et al. (2006) The Journal of biological chemistry 281, 11413-21. PMID: 16495226 9. Kalveram, B. et al. (2008) Journal of cell science 121, 4079-88. PMID: 19033385 10. Ross, MJ. et al. (2006) Journal of the American Society of Nephrology : JASN 17, 996-1004. PMID: 16495380
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human UBD Protein, N-His

References

HT365012-SDS-PAGE-1.jpg

Datasheet

Human UBD Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Human UBD Recombinant Protein (N-His) (HT365012)

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