Anti-UCHL1 Polyclonal Antibody (HY589014)

Name: Anti-UCHL1 Polyclonal Antibody
Brand: abinScience
SKU: HY589014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY589014

Description

Anti-UCHL1 Polyclonal Antibody (HY589014) is a rabbit polyclonal antibody detecting UCHL1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Equus caballus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human UCHL1 (Gln2-Ala223).

Target

UCH-L1, PGP 9.5, UCHL1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP9.5

Purification

Purified by antigen affinity column.

Accession

P09936

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a ~24 kDa protein. Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes.

1. Zhang, M. et al. (2012) Journal of neurochemistry 120, 1129-38. PMID: 22212137

2. Bilguvar, K. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 3489-94. PMID: 23359680

3. Goto, Y. et al. (2015) Nature communications 6, 6153. PMID: 25615526

4. Liu, Y. et al. (2002) Cell 111, 209-18. PMID: 12408865

5. Larsen, CN. et al. (1996) Biochemistry 35, 6735-44. PMID: 8639624

6. Leroy, E. et al. (1998) Nature 395, 451-2. PMID: 9774100

Note

For research use only.

Images

References

Datasheet

Anti-UCHL1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-UCHL1 Polyclonal Antibody (HY589014)

Overview

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References

Datasheet

SDS

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