Human UCHL1 Recombinant Protein (N-His) (HY589012)

Name: Human UCHL1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY589012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY589012
Description	Recombinant Human UCHL1 Protein, N-His (HY589012) expressed in E. coli, spanning Gln2-Ala223. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P09936
Protein length	Gln2-Ala223
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	26.99 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	UCH-L1, PGP 9.5, UCHL1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP9.5
Background	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a ~24 kDa protein. Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. 1. Zhang, M. et al. (2012) Journal of neurochemistry 120, 1129-38. PMID: 22212137 2. Bilguvar, K. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 3489-94. PMID: 23359680 3. Goto, Y. et al. (2015) Nature communications 6, 6153. PMID: 25615526 4. Liu, Y. et al. (2002) Cell 111, 209-18. PMID: 12408865 5. Larsen, CN. et al. (1996) Biochemistry 35, 6735-44. PMID: 8639624 6. Leroy, E. et al. (1998) Nature 395, 451-2. PMID: 9774100
Note	For research use only.

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SDS-PAGE for Recombinant Human UCHL1 Protein, N-His

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Datasheet

Human UCHL1 Recombinant Protein (N-His).pdf

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Human UCHL1 Recombinant Protein (N-His) (HY589012)

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