Anti-WWTR1 Polyclonal Antibody (HV017014)

Name: Anti-WWTR1 Polyclonal Antibody
Brand: abinScience
SKU: HV017014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV017014

Description

Anti-WWTR1 Polyclonal Antibody (HV017014) is a rabbit polyclonal antibody detecting WWTR1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WWTR1 (Met1-Leu400).

Target

Transcriptional coactivator with PDZ-binding motif, TAZ, WW domain-containing transcription regulator protein 1, WWTR1

Purification

Purified by antigen affinity column.

Accession

Q9GZV5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

WW domain-containing transcription regulator protein 1 (WWTR1) is a ~44 kDa protein. Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex.

1. Kanai, F. et al. (2000) The EMBO journal 19, 6778-91. PMID: 11118213

2. Lei, QY. et al. (2008) Molecular and cellular biology 28, 2426-36. PMID: 18227151

3. Lucci, V. et al. (2013) Gene 529, 300-6. PMID: 23911299

4. Di Palma, T. et al. (2009) Experimental cell research 315, 162-75. PMID: 19010321

5. Varelas, X. et al. (2008) Nature cell biology 10, 837-48. PMID: 18568018

Note

For research use only.

Images

References

Datasheet

Anti-WWTR1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-WWTR1 Polyclonal Antibody (HV017014)

Overview

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References

Datasheet

SDS

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