Human WWTR1 Recombinant Protein (N-His) (HV017012)

Name: Human WWTR1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV017012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV017012
Description	Recombinant Human WWTR1 Protein, N-His (HV017012) expressed in E. coli, spanning Met1-Leu400. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9GZV5
Protein length	Met1-Leu400
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	46.26 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Transcriptional coactivator with PDZ-binding motif, TAZ, WW domain-containing transcription regulator protein 1, WWTR1
Background	WW domain-containing transcription regulator protein 1 (WWTR1) is a ~44 kDa protein. Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex. 1. Kanai, F. et al. (2000) The EMBO journal 19, 6778-91. PMID: 11118213 2. Lei, QY. et al. (2008) Molecular and cellular biology 28, 2426-36. PMID: 18227151 3. Lucci, V. et al. (2013) Gene 529, 300-6. PMID: 23911299 4. Di Palma, T. et al. (2009) Experimental cell research 315, 162-75. PMID: 19010321 5. Varelas, X. et al. (2008) Nature cell biology 10, 837-48. PMID: 18568018
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human WWTR1 Protein, N-His

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HV017012-SDS-PAGE-1.jpg

Datasheet

Human WWTR1 Recombinant Protein (N-His).pdf

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Human WWTR1 Recombinant Protein (N-His) (HV017012)

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