HEK293T Human CD10/MME Stable Cell Line (HY401818)

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Overview

Catalog No.	HY401818
Description	HEK293T Human CD10/MME Stable Cell Line (HY401818) is a stable cell line expressing CD10/MME. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P08473
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD10/MME
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). 1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675 2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535 3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683 4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535 5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991 6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD10 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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