HEK293T Human CD193/CCR3 Stable Cell Line (HW620818)

Datasheet Share product Copy info

Overview
Images
References
Datasheet

Overview

Catalog No.	HW620818
Description	HEK293T Human CD193/CCR3 Stable Cell Line (HW620818) is a stable cell line expressing CD193/CCR3. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P51677
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD193/CCR3
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	C-C chemokine receptor type 3 (CD193/CCR3) is a ~41 kDa protein. G protein-coupled receptor (GPCR) that plays a key role in the immune system by regulating the migration and activation of white blood cells in response to chemokines. Selectively interacts with eosinophil-attracting chemokines such as eotaxin/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26. Ligand binding triggers intracellular signaling that leads to chemotaxis of immune cells. Mechanistically, signals through GNA14 or GNA16 to induce stimulation of phospholipase Cbeta/PLCB2 and subsequently chemotaxis. Alternatively, transduces signal via GNAI1 resulting in elevated intracellular calcium levels and activation of the PI3K/AKT pathway. 1. Song, Y. et al. (2018) Biochemistry 57, 852-860. PMID: 28994588 2. Combadiere, C. et al. (1995) The Journal of biological chemistry 270, 16491-4. PMID: 7622448 3. Daugherty, BL. et al. (1996) The Journal of experimental medicine 183, 2349-54. PMID: 8642344 4. Ponath, PD. et al. (1996) The Journal of experimental medicine 183, 2437-48. PMID: 8676064 5. Tian, Y. et al. (2008) Cellular signalling 20, 1179-89. PMID: 18406577 6. Shao, Z. et al. (2022) Cell discovery 8, 44. PMID: 35570218 7. Park, JS. et al. (2018) International journal of biological macromolecules 120, 835-845. PMID: 30171954

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD193 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

HEK293T Human CD193/CCR3 Stable Cell Line.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

HEK293T Human CD193/CCR3 Stable Cell Line (HW620818)

Overview

Images

References

Datasheet

Quick access

Contact Us