HEK293T Human CD203c/ENPP3 Stable Cell Line (HT018818)

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Overview

Catalog No.	HT018818
Description	HEK293T Human CD203c/ENPP3 Stable Cell Line (HT018818) is a stable cell line expressing CD203c/ENPP3. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	O14638
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD203c/ENPP3
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (CD203c/ENPP3) is a ~100 kDa protein. Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Also hydrolyzes extracellular 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production, and is therefore involved in the regulation of innate immune response. 2',3'-cGAMP appears to be further processed to GMP, AMP and inorganic phosphate. Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. 1. Gorelik, A. et al. (2018) The FEBS journal 285, 2481-2494. PMID: 29717535 2. Jin-Hua, P. et al. (1997) Genomics 45, 412-5. PMID: 9344668 3. Mardjuki, R. et al. (2024) Cell reports 43, 114209. PMID: 38749434 4. Vollmayer, P. et al. (2003) European journal of biochemistry 270, 2971-8. PMID: 12846830 5. Bühring, HJ. et al. (2001) Blood 97, 3303-5. PMID: 11342463

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human ENPP3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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