HEK293T Human CD344/FZD4 Stable Cell Line (HV376818)

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Datasheet

Overview

Catalog No.	HV376818
Description	HEK293T Human CD344/FZD4 Stable Cell Line (HV376818) is a stable cell line expressing CD344/FZD4. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	Q9ULV1
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD344/FZD4
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Frizzled-4 (CD344/FZD4) is a ~59 kDa protein. Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. 1. Yang, S. et al. (2018) Nature 560, 666-670. PMID: 30135577

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human FZD4 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

HEK293T Human CD344/FZD4 Stable Cell Line.pdf

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HEK293T Human CD344/FZD4 Stable Cell Line (HV376818)

Overview

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References

Datasheet

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