HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818)

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Overview

Catalog No.	HF614818
Description	HEK293T Human LGR5/GPR49 Stable Cell Line (HF614818) is a stable cell line expressing LGR5/GPR49. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	O75473
Applications	FCM
Species	Human
Host species	HEK293T
Target	LGR5/GPR49
Detection method	Colorimetric
Sample type	Plasma, Serum
Assay type	Quantitative
Range	1.56 - 100 ng/mL
Sensitivity	0.25 ng/mL
Recovery	80-120%
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Shipping	2-8 ℃
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is a ~99 kDa protein. Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Note	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human LGR5 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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