InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001) (HF827010)

Name: InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001)
Brand: abinScience
SKU: HF827010
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HF827010
Description	InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001) [Iv0001] (HF827010) is a human monoclonal antibody detecting Interferon alpha-2 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, WB, Neutralization
Host species	Human
Clonality	Monoclonal
Isotype	IgG1
Clone ID	Iv0001
Target	Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2, IFN-Alpha, IFNA2A, IFNA2B, IFNA2C, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFNA1, IFNA13, IFN-α4, IFN-α5, IFN-α6, IFN-α7, IFN-α8, IFN-αl0, IFN-α14, IFN-α16, IFN-α17, IFN-α21
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Associated products	pH 7.4 Dilution Buffer
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01563
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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Bioactivity

Detects Human IFN α2/IFNA2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IFN α2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IFN α2 antibody.SHSY-5Y cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001).
Western blot

Western blot analysis was performed using anti-IFNA2/IFN-alpha-2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 50ng
Lane 2: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 30ng
Lane 3: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 20ng
Lane 4: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 10ng

References

Datasheet

InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001).pdf

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Formula

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InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001) (HF827010)

Overview

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References

Datasheet

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