InVivoMAb Anti-Mouse IL-2 Antibody (JES6-1A12) (MX011010)

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Spec:

100ug
1mg

Number:

Overview
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References
Datasheet
SDS

Overview

Catalog No.

MX011010

Description

InvivoMab Anti-Mouse IL2 Antibody (JES6-1A12) [JES6-1A12] (MX011010) is a rat monoclonal antibody detecting Aldesleukin in ELISA, FCM. Suitable for Mouse.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Mouse

Applications

ELISA, FCM

Host species

Rat

Isotype

IgG2b, kappa

Clone ID

JES6-1A12

Clonality

Monoclonal

Target

Aldesleukin, IL-2, IL2, Interleukin-2, T-cell growth factor, TCGF

Endotoxin level

< 1 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P60568

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-2 (IL2) is a ~17 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.

1. Mingari, MC. et al. (1984) Nature 312, 641-3. PMID: 6438535

2. Wang, X. et al. (2005) Science (New York, N.Y.) 310, 1159-63. PMID: 16293754

3. Stauber, DJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2788-93. PMID: 16477002

4. Miyazaki, T. et al. (1994) Science (New York, N.Y.) 266, 1045-7. PMID: 7973659

5. Kirken, RA. et al. (1995) Cytokine 7, 689-700. PMID: 8580378

6. Osinalde, N. et al. (2015) Proteomics 15, 520-31. PMID: 25142963

7. Holbrook, NJ. et al. (1984) Proceedings of the National Academy of Sciences of the United States of America 81, 1634-8. PMID: 6608729

Note

For research use only.

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