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Normal Guinea pig IgG Recombinant Isotype Control Antibody (ZF690307)

Normal Guinea pig IgG Recombinant Isotype Control Antibody
Normal Guinea pig IgG Recombinant Isotype Control Antibody
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Spec:
  • 100ug
  • 1mg
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  • Overview

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  • Datasheet

  • SDS

Overview
Catalog No.ZF690307
Description
Normal Guinea pig IgG Recombinant Isotype Control Antibody (ZF690307) is a guinea pig polyclonal antibody detecting IgG in FCM, IHC, IF, Isotype Control. Suitable for Guinea pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityGuinea pig
ApplicationsFCM, IHC, IF, Isotype Control
Host speciesGuinea pig
ClonalityPolyclonal
IsotypeIgG
Nature Native
Endotoxin level < 10 EU/mg
Purity >90% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Immunoglobulin heavy constant gamma 4 (IgG4) is a ~43 kDa protein. Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain.

1. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170
2. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268
3. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414
Note For research use only.
Images
  • Normal Guinea pig IgG Recombinant Isotype Control Antibody

    SDS-PAGE

    SDS-PAGE for Normal Guinea pig IgG Isotype Control Antibody.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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