BK polyomavirus/BKPyV Major capsid protein VP1 Recombinant Protein (C-Strep) (VK452011)

Name: BK polyomavirus/BKPyV Major capsid protein VP1 Recombinant Protein (C-Strep)
Brand: abinScience
SKU: VK452011
Price: 264 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	VK452011
Description	Recombinant BK polyomavirus/BKPyV Major capsid protein VP1 Protein, C-Strep (VK452011) expressed in Insect Cells, spanning Met1-Val362. Purity: >90% by SDS-PAGE. Highlights ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Insect cells
Accession	P14996
Protein length	Met1-Val362
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	BK polyomavirus (strain AS) (BKPyV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	41.30 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Major capsid protein VP1, Major structural protein VP1
Background	Major capsid protein VP1 is a ~40 kDa protein. Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with gangliosides GT1b and GD1b containing terminal alpha(2-8)-linked sialic acids on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant BK polyomavirus/BKPyV Major capsid protein VP1 Protein, C-Strep
Bioactivity

Detected by Traxivitug (Catalog No: VK698016) in indirect ELISA.