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Feline Beta-NGF Recombinant Protein (C-Fc) (FF909011)

Feline Beta-NGF Recombinant Protein (C-Fc)
Feline Beta-NGF Recombinant Protein (C-Fc)
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  • 100ug
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Overview
Catalog No.FF909011
Description
Recombinant Cat NGF/Beta-NGF Protein, C-Fc (FF909011) expressed in Mammalian Cells, spanning Met19-Gly256. Purity: >90% by SDS-PAGE.
Highlights
  • ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Expression systemMammalian cells
AccessionQ6ZY27
Protein lengthMet19-Gly256
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesFelis catus (Cat) (Felis silvestris catus)
Nature Recombinant
Target Beta-NGF, Beta-nerve growth factor, NGF, NGFB
Endotoxin level <0.1 EU/ug as determined by LAL method.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 54.51 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesBeta-NGF, Beta-nerve growth factor, NGF, NGFB
Background

Beta-nerve growth factor (Beta-nerve growth factor,NGFB,Beta-NGF,NGF) is a ~26 kDa protein. Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI.

1. Einarsdottir, E. et al. (2004) Human molecular genetics 13, 799-805. PMID: 14976160
2. Carvalho, OP. et al. (2011) Journal of medical genetics 48, 131-5. PMID: 20978020
3. Wijeyewickrema, LC. et al. (2010) The Journal of biological chemistry 285, 11793-9. PMID: 20164177
Note For research use only
Images
  • Feline Beta-NGF Recombinant Protein (C-Fc)

    SDS-PAGE

    SDS-PAGE for Recombinant Cat NGF/Beta-NGF Protein, C-Fc

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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