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HCV Envelope glycoprotein E2 Recombinant Protein (C-Fc) (VK681021)

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Overview
Catalog No.VK681021
Description
Recombinant HCV Envelope Glycoprotein E2, C-Fc (VK681021) expressed in Mammalian Cells. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●High Purity — >90% as determined by SDS-PAGE.
Expression systemMammalian cells
AccessionP27958
Protein lengthAsp384-Ala708
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHepatitis C Virus (HCV)
Nature Recombinant
Target Envelope glycoprotein E2, NS1, gp68, gp70
Endotoxin level <0.1 EU/ug as determined by LAL method.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 64.57 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEnvelope glycoprotein E2, NS1, gp68, gp70
Background

Genome polyprotein is a ~327 kDa protein. Packages viral RNA to form a viral nucleocapsid, and promotes virion budding (Probable). Participates in the viral particle production as a result of its interaction with the non-structural protein 5A. Binds RNA and may function as a RNA chaperone to induce the RNA structural rearrangements taking place during virus replication (Probable). Modulates viral translation initiation by interacting with viral IRES and 40S ribosomal subunit. Affects various cell signaling pathways, host immunity and lipid metabolism (Probable).

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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