

| Catalog No. | VK539021 |
|---|---|
| Description |
Recombinant Hepatitis B virus (adr) S-HBsAg Protein (VK539021). Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | Mammalian cells |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Hepatitis B virus (HBV) |
| Nature | Recombinant |
| Endotoxin level | <0.1 EU/ug as determined by LAL method. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | L glycoprotein, L-HBsAg, LHB, Large S protein, Large envelope protein, Large surface protein, Major surface antigen, S |
| Background | Large envelope protein (HBsAg) is a ~42 kDa protein. The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. |
| Note | For research use only |
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