HTLV-1 Tax Recombinant Protein (N-His) (VK044012)

Name: HTLV-1 Tax Recombinant Protein (N-His)
Brand: abinScience
SKU: VK044012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK044012
Description	Recombinant HTLV-1 Tax Protein, N-His (VK044012) expressed in E. coli, spanning Gly26-Pro224. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03409
Protein length	Gly26-Pro224
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human T-cell leukemia virus 1 (HTLV-1)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.51 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein Tax-1, Protein X-LOR, Protein PX, Trans-activating transcriptional regulatory protein of HTLV-1, Tax
Background	Protein Tax-1 is a ~39 kDa protein. Transcriptional activator that governs the viral transcription from the 5'LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription. Increases host CREB1 O-GlcNAcylation to further increase 5'LTR transactivation. Modulates also the expression of cellular genes leading to the deregulation of T-cell proliferation, perturbing the integrity of cell cycle checkpoints, the DNA damage response and apopototic pathways. Acts as an ubiquitin E3 ligase and stimulates host IKK complex by catalyzing the assembly of free mixed-linkage polyubiquitin chains, resulting in constitutive activation of the transcription factor NF-kappa-B. 1. Kusano, S. et al. (2012) Virology 432, 343-52. PMID: 22789739 2. Tie, F. et al. (1996) Journal of virology 70, 8368-74. PMID: 8970957 3. Groussaud, D. et al. (2017) PLoS pathogens 13, e1006518. PMID: 28742148 4. Wang, C. et al. (2016) PLoS pathogens 12, e1005584. PMID: 27082114 5. Shibata, Y. et al. (2017) PLoS pathogens 13, e1006162. PMID: 28103322
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant HTLV-1 Tax Protein, N-His

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Datasheet

HTLV-1 Tax Recombinant Protein (N-His).pdf

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HTLV-1 Tax Recombinant Protein (N-His) (VK044012)

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