Human ABCA3 Recombinant Protein (N-His) (HV604012)

Name: Human ABCA3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV604012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV604012
Description	Recombinant Human ABCA3 Protein, N-His (HV604012) expressed in E. coli, spanning Asp1358-Phe1635. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q99758
Protein length	Asp1358-Phe1635
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	32.85 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ABCA3, ATP-binding cassette sub-family A member 3, Phospholipid-transporting ATPase ABCA3, ABC3, ATP-binding cassette 3, ATP-binding cassette transporter 3, ABC-C transporter, Xenobiotic-transporting ATPase ABCA3
Background	Phospholipid-transporting ATPase ABCA3 is a ~191 kDa protein. Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity. 1. Matsumura, Y. et al. (2006) The Journal of biological chemistry 281, 34503-14. PMID: 16959783 2. Matsumura, Y. et al. (2007) FEBS letters 581, 3139-44. PMID: 17574245 3. Wittmann, T. et al. (2016) Pediatric pulmonology 51, 1284-1294. PMID: 27177387 4. Höppner, S. et al. (2017) Biochimica et biophysica acta. Molecular cell research 1864, 2330-2335. PMID: 28887056 5. Li, Y. et al. (2019) Biochimica et biophysica acta. Molecular and cell biology of lipids 1864, 158516. PMID: 31473345 6. Zarbock, R. et al. (2015) Biochimica et biophysica acta 1851, 987-95. PMID: 25817392 7. Dohmen, LC. et al. (2016) The Journal of biological chemistry 291, 9638-47. PMID: 26903515
Note	For research use only.

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SDS-PAGE for Recombinant Human ABCA3 Protein, N-His

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Datasheet

Human ABCA3 Recombinant Protein (N-His).pdf

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Human ABCA3 Recombinant Protein (N-His) (HV604012)

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