ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
22.05 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
pp32, Putative HLA-DR-associated protein I, MAPM, ANP32A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Mapmodulin, LANP, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, PHAPI, Leucine-rich acidic nuclear protein, Acidic nuclear phosphoprotein pp32, PHAP1
Background
Acidic leucine-rich nuclear phosphoprotein 32 family member A (ANP32A) is a ~28 kDa protein. Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription. Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation. In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking. Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition.
1. Brody, JR. et al. (1999) The Journal of biological chemistry 274, 20053-5. PMID: 10400610
2. Bai, J. et al. (2001) Oncogene 20, 2153-60. PMID: 11360199
3. Fan, Z. et al. (2006) Cell death and differentiation 13, 1485-94. PMID: 16341127
4. Kim, HE. et al. (2008) Molecular cell 30, 239-47. PMID: 18439902
5. Seo, SB. et al. (2002) The Journal of biological chemistry 277, 14005-10. PMID: 11830591
6. Mazroui, R. et al. (2008) The Journal of cell biology 180, 113-27. PMID: 18180367
7. Tsujio, I. et al. (2005) FEBS letters 579, 363-72. PMID: 15642345
8. Cvetanovic, M. et al. (2007) EMBO reports 8, 671-7. PMID: 17557114
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human ANP32A Protein, N-His
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