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Human ARL1 Recombinant Protein (N-His) (HD512012)

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Overview
Catalog No.HD512012
Description
Recombinant Human ARL1 Protein, N-His (HD512012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP40616
Protein lengthMet1-Gln181
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesADP-ribosylation factor-like protein 1, ARL1
Background

ADP-ribosylation factor-like protein 1 is a ~20 kDa protein. GTP-binding protein that recruits several effectors, such as golgins, arfaptins and Arf-GEFs to the trans-Golgi network, and modulates their functions at the Golgi complex. Plays thereby a role in a wide range of fundamental cellular processes, including cell polarity, innate immunity, or protein secretion mediated by arfaptins, which were shown to play a role in maintaining insulin secretion from pancreatic beta cells.

1. Man, Z. et al. (2011) The Journal of biological chemistry 286, 11569-78. PMID: 21239483
2. Nakamura, K. et al. (2012) The Journal of biological chemistry 287, 25478-89. PMID: 22679020
3. Galindo, A. et al. (2016) Cell reports 16, 839-50. PMID: 27373159
4. Wang, R. et al. (2016) Journal of molecular cell biology 8, 459-461. PMID: 27436755
5. Hong, JX. et al. (1998) The Journal of biological chemistry 273, 15872-6. PMID: 9624189
6. Lu, L. et al. (2003) Molecular biology of the cell 14, 3767-81. PMID: 12972563
7. Gehart, H. et al. (2012) Developmental cell 23, 756-68. PMID: 22981988
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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