Human B4GALNT2 Recombinant Protein (N-His) (HP667012)

Name: Human B4GALNT2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP667012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP667012
Description	Recombinant Human B4GALNT2 Protein, N-His (HP667012) expressed in E. coli, spanning Thr324-Ala566. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q8NHY0
Protein length	Thr324-Ala566
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.92 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Beta-1, 4 N-acetylgalactosaminyltransferase 2, B4GALNT2, GALGT2, Sd(a) beta-1, 4-GalNAc transferase, UDP-GalNAc:Neu5Aca2-3Galb-R b1, 4-N-acetylgalactosaminyltransferase
Background	Beta-1,4 N-acetylgalactosaminyltransferase 2 (B4GALNT2) is a ~63 kDa protein. Beta-1,4 N-acetylgalactosaminyltransferase involved in the biosynthesis of Sd(a) histo-blood group antigen. Catalyzes the transfer of N-acetylgalactosamine (GalNAc) group in a beta-1,4-linkage from UDP-GalNAc to the galactose residue of NeuAcalpha2->3Gal-R to form Sd(a) glycan epitope GalNAcbeta1->4(NeuAcalpha2->3)Gal-R. The Sd(a) epitope is carried in O- and N-linked glycoproteins and glycolipids, including O-linked core 1 structures on GYPA/glycophorin, SLC4A1 and SLC29A1 in erythrocytes, N-linked glycans attached to the Tamm-Horsfall glycoprotein UMOD/uromodulin in renal fluids, O-linked core 3 glycans on mucins in colon and neolactosides in gastric mucosa. Confers protection against influenza A virus strains that attach to NeuAcalpha2->3-carrying host receptors. Modifies N-glycan chains on host receptors and prevents virus entry into cells. 1. Montiel, MD. et al. (2003) The Biochemical journal 373, 369-79. PMID: 12678917 2. Lo Presti, L. et al. (2003) Journal of biochemistry 134, 675-82. PMID: 14688233 3. Kawamura, YI. et al. (2005) Cancer research 65, 6220-7. PMID: 16024623 4. Stenfelt, L. et al. (2022) International journal of molecular sciences 23. PMID: 35409292 5. Heaton, BE. et al. (2017) Cell reports 20, 1503-1512. PMID: 28813663
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human B4GALNT2 Protein, N-His

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Datasheet

Human B4GALNT2 Recombinant Protein (N-His).pdf

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Human B4GALNT2 Recombinant Protein (N-His) (HP667012)

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