ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
25.83 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Hyaluronan-binding protein 1, SF2AP32, C1QBP, gC1q-R protein, SF2P32, Glycoprotein gC1qBP, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32, GC1QBP, HABP1, p33, C1qBP, Mitochondrial matrix protein p32
Background
Complement component 1 Q subcomponent-binding protein, mitochondrial (C1QBP) is a ~31 kDa protein. Multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, protein synthesis in mitochondria, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. At the cell surface is thought to act as an endothelial receptor for plasma proteins of the complement and kallikrein-kinin cascades. Putative receptor for C1q; specifically binds to the globular 'heads' of C1q thus inhibiting C1; may perform the receptor function through a complex with C1qR/CD93. In complex with cytokeratin-1/KRT1 is a high affinity receptor for kininogen-1/HMWK. Can also bind other plasma proteins, such as coagulation factor XII leading to its autoactivation.
1. Petersen-Mahrt, SK. et al. (1999) The EMBO journal 18, 1014-24. PMID: 10022843
2. Joseph, K. et al. (1999) Clinical immunology (Orlando, Fla.) 92, 246-55. PMID: 10479529
3. Nguyen, T. et al. (2000) Infection and immunity 68, 2061-8. PMID: 10722602
4. Kittlesen, DJ. et al. (2000) The Journal of clinical investigation 106, 1239-49. PMID: 11086025
5. Feng, X. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 2441-8. PMID: 11859136
6. Chattopadhyay, C. et al. (2004) Nucleic acids research 32, 3632-41. PMID: 15243141
7. Vegh, Z. et al. (2006) Molecular immunology 43, 1402-7. PMID: 16140380
8. Waggoner, SN. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 4706-14. PMID: 16177118
9. Waggoner, SN. et al. (2007) Journal of leukocyte biology 82, 1407-19. PMID: 17881511
10. Huang, L. et al. (2008) Investigative ophthalmology & visual science 49, 5243-9. PMID: 18676636
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human C1QBP Protein, N-His
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