Human CALM1 Recombinant Protein (N-His) (HY390012)

Name: Human CALM1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY390012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY390012
Description	Recombinant Human CALM1 Protein, N-His (HY390012) expressed in E. coli, spanning Ala2-Lys149. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0DP23
Protein length	Ala2-Lys149
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CAM1, CALM1, CAM, CALM, Calmodulin-1
Background	Calmodulin-1 (CALM1) is a ~16 kDa protein. Calmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Calcium-binding is required for the activation of calmodulin. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases, such as myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Is a regulator of voltage-dependent L-type calcium channels. 1. Tsang, WY. et al. (2006) Molecular biology of the cell 17, 3423-34. PMID: 16760425 2. Reichow, SL. et al. (2013) Nature structural & molecular biology 20, 1085-92. PMID: 23893133 3. Boczek, NJ. et al. (2016) Circulation. Cardiovascular genetics 9, 136-146. PMID: 26969752 4. Yu, CC. et al. (2016) Heart rhythm 13, 1716-23. PMID: 27165696 5. Zhao, YG. et al. (2017) Molecular cell 67, 974-989.e6. PMID: 28890335 6. Wren, LM. et al. (2019) Circulation. Genomic and precision medicine 12, 375-385. PMID: 31454269 7. Alphonse, N. et al. (2022) Cell 185, 2354-2369.e17. PMID: 35568036
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CALM1 Protein, N-His

References

HY390012-SDS-PAGE-1.jpg

Datasheet

Human CALM1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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V₂ (Working Vol.)

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Human CALM1 Recombinant Protein (N-His) (HY390012)

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