ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
10.98 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2
Background
C-C motif chemokine 2 (CCL2/MCP-1) is a ~11 kDa protein. Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
1. Hemmerich, S. et al. (1999) Biochemistry 38, 13013-25. PMID: 10529171
2. Jarnagin, K. et al. (1999) Biochemistry 38, 16167-77. PMID: 10587439
3. Paavola, CD. et al. (1998) The Journal of biological chemistry 273, 33157-65. PMID: 9837883
4. Zhang, YJ. et al. (1994) The Journal of biological chemistry 269, 15918-24. PMID: 8195247
5. Weber, M. et al. (1996) The Journal of experimental medicine 183, 681-5. PMID: 8627182
6. Chakravarty, L. et al. (1998) The Journal of biological chemistry 273, 29641-7. PMID: 9792674
7. Li, YS. et al. (1993) Molecular and cellular biochemistry 126, 61-8. PMID: 8107690
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CCL2/MCP-1 Protein, N-His
Bioactivity
Detects Carlumab in indirect ELISAs.
Bioactivity
Detects Human CCL2/MCP-1 Antibody (11k2) in indirect ELISAs.
Western blot
Detected by Anti-CCL2/MCP-1 monoclonal antibody (Catalog No: HY218026)
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