ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
34.67 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
TCP228, C-C motif chemokine 5, CCL5, T cell-specific protein P228, D17S136E, Small-inducible cytokine A5, SIS-delta, SCYA5, T-cell-specific protein RANTES, Eosinophil chemotactic cytokine, EoCP
Background
C-C motif chemokine 5 (CCL5/RANTES) is a ~9 kDa protein. Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
1. Kameyoshi, Y. et al. (1992) The Journal of experimental medicine 176, 587-92. PMID: 1380064
2. Lim, JK. et al. (2005) Journal of leukocyte biology 78, 442-52. PMID: 15923218
3. Capoulade-Métay, C. et al. (2006) Immunogenetics 58, 533-41. PMID: 16791620
4. Cocchi, F. et al. (1995) Science (New York, N.Y.) 270, 1811-5. PMID: 8525373
5. Proost, P. et al. (1998) The Journal of biological chemistry 273, 7222-7. PMID: 9516414
6. Liu, B. et al. (2013) Diabetologia 56, 2467-76. PMID: 23979485
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CCL5/RANTES Protein, N-GST
Bioactivity
Detects lv0079 in indirect ELISAs.
Bioactivity
Detected by InVivoMAb Anti-Human CCL5/RANTES (Iv0079) (Cat No.: HY346010) in indirect ELISA.
Western blot
Detected by Anti-CCL5/RANTES monoclonal antibody (Catalog No: HY346107)
Bioactivity
Detected by Anti-Human CCL5/RANTES Antibody (SAA0456) (Catalog No: HY346107) in indirect ELISA
中文
English
한국어
日本語
Español
Français
Русский
