Human CD5L Recombinant Protein (N-His) (HT064012)

Name: Human CD5L Recombinant Protein (N-His)
Brand: abinScience
SKU: HT064012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT064012
Description	Recombinant Human CD5L Protein, N-His (HT064012) expressed in E. coli, spanning Pro133-Arg256. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O43866
Protein length	Pro133-Arg256
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.09 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, IgM-associated peptide, CT-2, hAIM, API6, SP-alpha, CD5L
Background	CD5 antigen-like (CD5L) is a ~38 kDa protein. Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). 1. Amézaga, N. et al. (2014) Journal of leukocyte biology 95, 509-20. PMID: 24295828 2. Sarrias, MR. et al. (2005) The Journal of biological chemistry 280, 35391-8. PMID: 16030018 3. Sanjurjo, L. et al. (2013) PloS one 8, e79670. PMID: 24223991 4. Martinez, VG. et al. (2014) Cellular & molecular immunology 11, 343-54. PMID: 24583716 5. Sanjurjo, L. et al. (2015) Autophagy 11, 487-502. PMID: 25713983 6. Arai, S. et al. (2016) Nature medicine 22, 183-93. PMID: 26726878
Note	For research use only.

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SDS-PAGE for Recombinant Human CD5L Protein, N-His

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Datasheet

Human CD5L Recombinant Protein (N-His).pdf

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Human CD5L Recombinant Protein (N-His) (HT064012)

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