ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
22.11 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1
Background
CD81 antigen is a ~25 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells. Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production. In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype. Present in MHC class II compartments, may also play a role in antigen presentation.
1. Shoham, T. et al. (2006) Molecular and cellular biology 26, 1373-85. PMID: 16449649
2. van Zelm, MC. et al. (2010) The Journal of clinical investigation 120, 1265-74. PMID: 20237408
3. Zimmerman, B. et al. (2016) Cell 167, 1041-1051.e11. PMID: 27881302
4. Cherukuri, A. et al. (2004) The Journal of biological chemistry 279, 31973-82. PMID: 15161911
5. Sagi, Y. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 1613-8. PMID: 22307619
6. Rocha-Perugini, V. et al. (2013) Molecular and cellular biology 33, 3644-58. PMID: 23858057
7. Secrist, H. et al. (1996) European journal of immunology 26, 1435-42. PMID: 8766544
8. Schick, MR. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 4090-7. PMID: 8409388
9. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD81 Protein, N-His-SUMO
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