Human CEP290 Recombinant Protein (N-His) (HT321012)

Name: Human CEP290 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT321012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT321012
Description	Recombinant Human CEP290 Protein, N-His (HT321012) expressed in E. coli, spanning Thr1289-Arg1416. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15078
Protein length	Thr1289-Arg1416
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.89 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Nephrocystin-6, KIAA0373, CEP290, Bardet-Biedl syndrome 14 protein, Tumor antigen se2-2, BBS14, NPHP6, Centrosomal protein of 290 kDa, Cancer/testis antigen 87, Cep290, CT87
Background	Centrosomal protein of 290 kDa (CEP290) is a ~290 kDa protein. Involved in early and late steps in cilia formation. Its association with CCP110 is required for inhibition of primary cilia formation by CCP110. May play a role in early ciliogenesis in the disappearance of centriolar satellites and in the transition of primary ciliar vesicles (PCVs) to capped ciliary vesicles (CCVs). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1. Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes. 1. Tsang, WY. et al. (2008) Developmental cell 15, 187-97. PMID: 18694559 2. Kobayashi, T. et al. (2014) The Journal of cell biology 204, 215-29. PMID: 24421332 3. Frank, V. et al. (2008) Human mutation 29, 45-52. PMID: 17705300 4. Barbelanne, M. et al. (2015) Human molecular genetics 24, 2185-200. PMID: 25552655 5. Sayer, JA. et al. (2006) Nature genetics 38, 674-81. PMID: 16682973
Note	For research use only.

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SDS-PAGE for Recombinant Human CEP290 Protein, N-His

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Datasheet

Human CEP290 Recombinant Protein (N-His).pdf

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Human CEP290 Recombinant Protein (N-His) (HT321012)

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