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Human CHRNB2 Recombinant Protein (N-His) (HB048012)

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Human CHRNB2 Recombinant Protein (N-His)
Human CHRNB2 Recombinant Protein (N-His)
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Overview
Catalog No.HB048012
Description
Recombinant Human CHRNB2 Protein, N-His (HB048012) expressed in E. coli, spanning Trp24-Pro244. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionP17787
Protein lengthTrp24-Pro244
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 28.3 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCHRNB2, Neuronal acetylcholine receptor subunit beta-2
Background

Neuronal acetylcholine receptor subunit beta-2 (CHRNB2) is a ~57 kDa protein. Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. CHRNB2 forms heteropentameric neuronal acetylcholine receptors with CHRNA2, CHRNA3, CHRNA4 and CHRNA6, as well as CHRNA5 and CHRNB3 as accesory subunits. Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation.

1. Bondarenko, V. et al. (2012) Biochimica et biophysica acta 1818, 1261-8. PMID: 22361591
2. Morales-Perez, CL. et al. (2016) Nature 538, 411-415. PMID: 27698419
3. Walsh, RM. et al. (2018) Nature 557, 261-265. PMID: 29720657
4. Mazzaferro, S. et al. (2024) British journal of pharmacology 181, 1973-1992. PMID: 38454578
5. Tumkosit, P. et al. (2006) Molecular pharmacology 70, 1358-68. PMID: 16835356
6. Kuryatov, A. et al. (2011) Molecular pharmacology 79, 119-25. PMID: 20881005
7. Wang, F. et al. (1996) The Journal of biological chemistry 271, 17656-65. PMID: 8663494
8. George, AA. et al. (2021) The Journal of neuroscience : the official journal of the Society for Neuroscience 41, 555-575. PMID: 33239400
Note For research use only.
Images
  • Human CHRNB2 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CHRNB2 Protein, N-His

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Human CHRNB2 Recombinant Protein (N-His)

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